The Museum of Saint Helena is a museum on the island of Saint Helena, a British Overseas Territory in the south Atlantic Ocean.

The museum is managed by the Saint Helena Heritage Society. It is housed in a late-18th-century stone building, formerly the old power station, at the foot of the hillside stairway, Jacob’s Ladder, in lower Jamestown, Saint Helena’s capital. It was officially opened on 21 May 2002, the quincentennial anniversary of the island’s discovery, by the Governor, David Hollamby.

The museum is one of two on the island, the other being Longwood House, the house that Napoleon Bonaparte stayed in during his incarceration on St Helena.